Regulation of surfactant protein D in the mouse female reproductive tract in vivo.

نویسندگان

  • Rebecca E Oberley
  • Kelli L Goss
  • Darren S Hoffmann
  • Kevin A Ault
  • Traci L Neff
  • Kyle H Ramsey
  • Jeanne M Snyder
چکیده

Surfactant protein D (SP-D) plays a role in innate immunity in the lung and is expressed at many other mucosal surfaces throughout the human body. In this study, we show that SP-D mRNA and protein are present in the murine female reproductive tract; i.e. in the vagina, cervix, uterus and oviduct. SP-D protein is primarily localized to epithelial cells lining the genital tract and is also present in secretory material within the lumen of the uterus and cervix. The levels of SP-D mRNA in the uterus vary by a factor of 10 during the estrous cycle with peak levels present at estrus and the lowest levels at diestrus. In contrast, SP-D mRNA levels in the lung do not change during the estrous cycle. Since SP-D is an innate host defense protein present in the mouse reproductive tract, we studied the influence of infection on SP-D levels in vivo. We found that Chlamydia muridarum infection caused an increase in the SP-D protein content of reproductive tract epithelial cells. These data are suggestive that SP-D may play a role in innate immunity in the female reproductive tract in vivo.

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عنوان ژورنال:
  • Molecular human reproduction

دوره 13 12  شماره 

صفحات  -

تاریخ انتشار 2007